Journal: Scientific Reports

Article Title: New perspectives for viability studies with high-content analysis Raman spectroscopy (HCA-RS)

doi: 10.1038/s41598-019-48895-7

Figure Lengend Snippet: The experimental setup of HCA-RS system has many components. They are ES – excitation source, F – fiber, L – lens, CF – clean-up filter, EF – edge filter, M – mirror, OBJ – objective, STG – xyz stages, LP – long pass filter, BF – bright field camera, LED – light emitting diode, SPC – spectrograph and CCD – Raman spectral detector. The cartridge is placed on the STG and can accommodate six CaF 2 coverslips for six different measurements in one run. The light green, the purple and the pink lines indicate the excitation, the Stokes Raman signal and the bright field illumination path, respectively. In this work we referred Stokes Raman as Raman signal which is generated in the sample plane and travels back with the excitation path up to EF1 and is then separated (along the light green line from the sample plane up to the EF1).

Article Snippet: Both of the scattered signal from the sample are reflected back, guided by the mirror M1 and then they propagate through the edge filter EF1 (SEMROCK, USA) allowing only the Stokes Raman signal to pass, then through a low pass filter LP (SEMROCK, USA) and lastly another edge filter EF2 (SEMROCK, USA).

Techniques: Generated